Live Olympia oysters

Checked on leftover Olys from my 2017 and 2018 Manchester experiments yesterday (6/16/19) which were housed at Manchester on the beach and dock.

Beach Status

In June 2018 I put mini silos with Oly set - 1 from each “family” - into 3 big oyster bags, and Stuart put them onto the beach in Clam Bay, where they stayed for 12 months. I had cut the silo screen out, so there was plenty of waterflow. Yesterday I walked out at low tide and collected the bags. They were shockingly clean - just some kelp growing on the top side of the bag. I only found a few barnacles set on silos. Very few Olys survived:

[hold for data table]

In one of the bags I had placed a HOBO pendant data logger. Here’s a snapshot of the temperature (black) and light intensity (blue) data:

Dock Status

I have 3 cages of Olys hanging from the dock, which contain

a) Remnant F1 Olys from Jake Heare, which I used in my OA/T experiment and exposed to varying temp and pCO2 levels (probably includes soem of of Katherine’s Oyster Bay F2’s, too).

b) Offspring from my Oly OA/T 2017-2018 experiment, bagged into window screen pouches. Not sure how many I have of each group, but there were tons of live oysters.

c) Offspring from my Oly temperature/food 2018 experiment. They were bagged into 450um mesh silos, and had now grown much at all. They were alive, though!

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Planning for final QuantSeq run

Sample inventory is located here

Need to identify how many samples I have for the large QuantSeq run. I want to do juveniles that were deployed in Port Gamble Bay, Puget Sound. Here’s the inventory:

BAY	HABITAT	# Samples	COHORT	Parental pCO2 treatment	TISSUE TYPE (whole, gill, gut)
Port Gamble	Bare	5	Dabob Bay	high	whole body
Port Gamble	Bare	4	Dabob Bay	ambient	whole body
Port Gamble	Eelgrass	5	Dabob Bay	high	whole body
Port Gamble	Eelgrass	5	Dabob Bay	ambient	whole body
Port Gamble	Bare	5	Fidalgo Bay	high	whole body
Port Gamble	Bare	0	Fidalgo Bay	ambient	whole body
Port Gamble	Eelgrass	5	Fidalgo Bay	high	whole body
Port Gamble	Eelgrass	5	Fidalgo Bay	ambient	whole body
Port Gamble	Bare	4	Oyster Bay C1	high	whole body
Port Gamble	Eelgrass	5	Oyster Bay C1	ambient	whole body
Port Gamble	Bare	3	Dabob Bay	high	gill
Port Gamble	Eelgrass	3	Dabob Bay	ambient	gill
Port Gamble	Eelgrass	3	Dabob Bay	high	gill
Port Gamble	Bare	3	Fidalgo Bay	high	gill
Port Gamble	Eelgrass	3	Fidalgo Bay	ambient	gill
Port Gamble	Eelgrass	3	Fidalgo Bay	high	gill

Larval samples collected spring 2017 from OA/T adults

Below is status of all the frozen larvae I sampled day of release, pooled by spawn bucket.

Cohort	Treatment (T, pH)	# samples collected	# samples pristine
Dabob Bay	10 Ambient	4	4
Dabob Bay	10 Low	5	2
Dabob Bay	6 Ambient	1	0
Dabob Bay	6 Low	3	3
Fidalgo Bay	10 Ambient	5	5
Fidalgo Bay	10 Low	3	3
Fidalgo Bay	6 Ambient	2	2
Fidalgo Bay	6 Low	7	7
Oyster Bay C1	10 Ambient	13	13
Oyster Bay C1	10 Low	8	0
Oyster Bay C1	6 Ambient	7	0
Oyster Bay C1	6 Low	10	10
Oyster Bay C2	10 Ambient	3	3
Oyster Bay C2	10 Low	3	3
Oyster Bay C2	6 Ambient	4	4
Oyster Bay C2	6 Low	5	5