2021
February 2021
- O. lurida QuantSeq - initial analysis • February 2, 2021
2020
</ul>December 2020
- C. magister MiSeq analysis continued • December 27, 2020
- C. magister MiSeq data processing • December 15, 2020
- Oly methylation analysis, some general characteristic questions • December 8, 2020
November 2020
- Methylation Island Functional Analysis • November 30, 2020
May 2020
- Oly RNA - Data processing test-run • May 18, 2020
April 2020
- Oly OA RNA isolation - raw data • April 23, 2020
- Oly OA RNA isolation - sequencing • April 23, 2020
- April 23, 2020 - Oly methylation characterization • April 23, 2020
March 2020
- Oly OA RNA isolation - juvenile whole body continued • March 14, 2020
- Oly OA RNA isolation - juvenile whole-body • March 6, 2020
January 2020
- Oly methylation analysis, Jan 31st, 2020 • January 31, 2020
- Oly DMG analysis, Jan. 30th, 2020 • January 30, 2020
- February 2020 goals • January 30, 2020
- Oly methylation analysis, Jan. 29th 2020 • January 29, 2020
- QuantSeq - inventory of finished libraries • January 6, 2020
- QuantSeq library amplification, purification, and QA • January 4, 2020
2019
</ul>December 2019
- QuantSeq Library Generation Batch 5 • December 15, 2019
- QuantSeq Library Generation Batches 3 and 4 • December 14, 2019
- QuantSeq Purification and qPCR Assay Batches 3, 4, 5 (larvae) • December 10, 2019
- QuantSeq Purification and qPCR Assay Batches 1 & 2 • December 10, 2019
- QuantSeq Library Generation Batch 2 • December 6, 2019
- QuantSeq Library Generation Batch 1 • December 5, 2019
November 2019
- Oly methylation analysis, Nov 20, 2019 • November 20, 2019
October 2019
- November 2019 goals • October 27, 2019
September 2019
- Running list of R tips from super-advanced class • September 30, 2019
- Temperature data from Clam Bay, Mud Bay, Fidalgo Bay • September 13, 2019
- Analyzing MACAU results, take 3 • September 12, 2019
- Analyzing MACAU results, take 2 • September 7, 2019
- Analysis of QuantSeq Larval Sizes • September 6, 2019
- September, 2019 Goals • September 4, 2019
- QuantSeq Library Prep test-run • September 3, 2019
August 2019
- Measuring QuantSeq larvae • August 23, 2019
- Checking RNA quality with Bioanalyzer • August 21, 2019
- qPCR with Actin primer set, etc. • August 15, 2019
- Mid-August, 2019 goals & timeline • August 13, 2019
- Testing more Oly primers for DNA contamination • August 12, 2019
- MACAU Run 1 • August 12, 2019
- Prepping for QuantSeq library prep • August 12, 2019
- Learning about MACAU • August 7, 2019
- DNasing RNA, Round II-V • August 5, 2019
July 2019
- DNasing RNA, Round I • July 31, 2019
- Oly RNA isolation - larvae • July 27, 2019
- Oly OA RNA isolation - Ctenidia • July 16, 2019
- Starting RNA isolation for Oly OA project • July 10, 2019
June 2019
- June 2019 Animal Inventory • June 17, 2019
- June 2019 goals • June 9, 2019
May 2019
- May 2019 Goals • May 2, 2019
March 2019
- March goals • March 13, 2019
February 2019
- First day of tissue prep, Alanna captstone / acute OA shock trial • February 21, 2019
- February 2019 Goals • February 5, 2019
January 2019
- Oly OA gonad histology & spawning data options • January 28, 2019
- January 2019 Goals • January 12, 2019
2018
</ul>November 2018
- Assessing Oly gonad transcriptome assembly • November 8, 2018
October 2018
- Denovo Oly gonad transcriptome assembly (attempt 1) • October 29, 2018
- Survival data from Olympia oyster feeding/temp trial • October 24, 2018
- O. lurida RNAseq data fastqc & trim testing • October 23, 2018
September 2018
- October goals • September 27, 2018
- Histology processing protocol • September 27, 2018
August 2018
- August 2018 goals • August 1, 2018
July 2018
- July 18 2018, O. angasi conditioning • July 17, 2018
June 2018
- O. angasi heat shock trial • June 27, 2018
- Acute OA Exposure on 2017 Oly seed • June 1, 2018
May 2018
- Where are they now? • May 7, 2018
- May 2018 goals • May 7, 2018
April 2018
- Oly temp experiment, 29 days of larvae • April 27, 2018
- Whoa, April Goals • April 9, 2018
March 2018
- Oly larvae RNA isolation • March 30, 2018
- Oly gonad & larvae RNA isolation • March 27, 2018
- Final sampling pre-larvae • March 23, 2018
- Update on Olympia oyster temp/feeding experiment • March 15, 2018
- Assessing larval DNA integrity • March 14, 2018
- DNA Isolation from frozen Oly larvae • March 12, 2018
- Oly DNA Isolation, take 3 • March 9, 2018
February 2018
- Terminating 3-month Oly temp/food treatment • February 27, 2018
- Oly DNA Extraction, testing protocol • February 15, 2018
- (Mid-)February Goals • February 13, 2018
- An Observation, 2017 Oly Data • February 12, 2018
- Oly gonad statistics, 6-degree temperature only • February 12, 2018
- Prepping for DNA Extraction • February 12, 2018
- Gonad histology stats, all treatments • February 11, 2018
January 2018
- Oly Genetics 104, NF GenePop Analysis • January 22, 2018
- Oly Genetics 103, preparing microsat data for analysis in GenePop • January 22, 2018
- Oly Genetics Meeting Recap • January 22, 2018
- Oly Genetics 101 • January 17, 2018
- SRM Analysis, revisiting correlations between abundance & environment • January 12, 2018
- January 2018 goals • January 10, 2018
- DO Data from WA Ecology & DO Outlier Scrub re-do • January 3, 2018
2017
</ul>December 2017
- December Goals (belated) • December 12, 2017
November 2017
- Finalized list of SRM data analysis stats • November 28, 2017
- Initial look at Environmental Data • November 14, 2017
- Oyster New Year 2017 • November 11, 2017
- Olympia oyster gonad stage pie charts • November 7, 2017
- November Goals • November 7, 2017
- Collected FB Broodstock for Oly 2018 Temp. Experiment • November 6, 2017
October 2017
- Oly 2017 Exp Summary • October 26, 2017
- SRM data - quantifying tech. rep. quality • October 15, 2017
- Cleaning day - rinsing Olys and cleaning OA system • October 12, 2017
- Moved Olympia oyster seed to Manchester dock • October 4, 2017
September 2017
- Initial results from geoduck SRM data • September 28, 2017
- October Goals • September 27, 2017
August 2017
- Initial survival data, Olympia oyster experiment 2017 • August 28, 2017
- Video- collecting new Olympia oyster larvae • August 26, 2017
- Playing with SRM data • August 24, 2017
- Retention Time R2 Calcs • August 11, 2017
- Cleaning up SRM data in Skyline, part II • August 11, 2017
- My oysters are growing up • August 9, 2017
- Cleaning Srm Data In Skyline • August 8, 2017
- Oly spawning charts • August 3, 2017
July 2017
- Complete notes from mass spec run • July 28, 2017
- SRM Dilution Curve Sample Prep • July 24, 2017
- Remaining SRM Timeline • July 24, 2017
- Mass Spec, good things to know • July 24, 2017
- Mass spec hiccup? • July 21, 2017
- Second SRM sample prep • July 19, 2017
- Checking in on my oysters • July 19, 2017
- Predicting Retention Times to asses Assay Success • July 18, 2017
- SRM mass spec refresher, prepping samples with PRTC • July 17, 2017
- Geoduck SRM, desalting • July 9, 2017
- Manchester 7/8 • July 8, 2017
- Manchester, 7/5 & 7/6 • July 5, 2017
- Post Skyline Data Processing • July 4, 2017
- July Goals • July 4, 2017
- Manchester 7/3 • July 3, 2017
- Manchester, 7/1 • July 1, 2017
June 2017
- Manchester 6/28 & 6/29 • June 28, 2017
- Mancester 6/26 • June 26, 2017
- Manchester, 6/24 • June 24, 2017
- Manchester 6/23 • June 23, 2017
- Manchester 6/22 • June 22, 2017
- Manchester 6/20 & 6/21 • June 20, 2017
- Manchester, 6/19 • June 19, 2017
- Manchester, 6/18 • June 18, 2017
- Manchester, 6/16 & 6/17 • June 17, 2017
- Manchester, 6/15 • June 15, 2017
- Manchester, 6/13 & 6/14 • June 14, 2017
- Manchester, 6/12 • June 12, 2017
- Manchester, 6/10 • June 10, 2017
- Manchester, 6/9 • June 9, 2017
- Manchester 6-8 • June 8, 2017
- Manchester, 6/7 • June 7, 2017
- Manchester 6/5 • June 5, 2017
- Manchester, 5/31 to 6/4 • June 4, 2017
May 2017
- May 29th & 30th, at Manchester • May 30, 2017
- May 27th, 2017 • May 27, 2017
- May 23rd - 26th, 2017 • May 26, 2017
- May 22nd, 2017 • May 22, 2017
- May 21st 2017 • May 21, 2017
- Choosing the right ones. • May 20, 2017
- May 18th 2017 • May 18, 2017
- May 17th 2017 • May 17, 2017
- Downloaded T & pH Data • May 16, 2017
- An alternative look at proteins • May 16, 2017
- Interesting Proteins, part II • May 15, 2017
- Weekend Update with L. Spencer • May 14, 2017
- Spring Hatchlings • May 11, 2017
- Finding proteins of interest • May 11, 2017
- Spring cleaning • May 9, 2017
- planning next round of geoduck extractions • May 8, 2017
- Minor adjustments • May 4, 2017
- Avtech online! • May 3, 2017
- Oly's get new digs • May 2, 2017
- Check out those peaks! • May 2, 2017
- Using Skyline • May 2, 2017
- A first glimpse @ HOBO temperature data • May 1, 2017
April 2017
- Pre-spawn plumbing update • April 21, 2017
- preparing histology samples • April 20, 2017
- Pubathon - 14 Day Goals • April 17, 2017
- Demultiplexing & converting from .raw to .mzml • April 14, 2017
- Oly Sampling, Post-OA, Group K • April 13, 2017
- Re-Arranging Oly's for Conditioning • April 11, 2017
- Oly Sampling, post-OA • April 8, 2017
- Day 50 Activities • April 6, 2017
- April 2017 Goals • April 5, 2017
- Day 48 Activities • April 4, 2017
March 2017
- Day 36 Activities • March 23, 2017
- Day 33 Activities report • March 20, 2017
- Experimental Pecan Run using Gigas genome • March 17, 2017
- Day 28 Activities • March 15, 2017
- Pecan Again?! • March 13, 2017
- OA - Day 26 activities • March 13, 2017
- Chopping Pecan into little pieces • March 13, 2017
- Checking in on Pecan • March 7, 2017
- 3 weeks of acid • March 6, 2017
- OA Experiment, Day 19 • March 6, 2017
- Long Overdue Pecan Things • March 3, 2017
- Tank Swap! (etc.) • March 1, 2017
- March Goalz • March 1, 2017
February 2017
- Sneak Peak of Continuous Temperature data from the winter's Oly treatments • February 28, 2017
- OA Exp. Day 12 • February 27, 2017
- First weekly maintenance • February 22, 2017
- Two water chemistry sampling days • February 21, 2017
- A "stressful" afternoon • February 15, 2017
- OA Prep, devil's in the details • February 11, 2017
- Getting reaquainted with the OA system • February 7, 2017
- O. lurida go under the knife • February 5, 2017
- O. lurida Climate Stressor project well underway! • February 3, 2017
January 2017
- Lumos in action! • January 24, 2017
- Firing up the Mass Spec • January 23, 2017
- Random prep for Mass Spec • January 15, 2017
- Goals in the New Year • January 3, 2017
2016
</ul>December 2016
- Lab Day 6 - Desalting • December 14, 2016
- Lab day 5 - Evaporation • December 13, 2016
- Lab day 4 - Digestion • December 13, 2016
- Lab day 3 - Protein Quantification via BCA Assay • December 12, 2016
- Lab day 2 - Homogenization & Sonication • December 9, 2016
- Lab day 1 - Make reagents, divide sample, prep • December 7, 2016
November 2016
- November 2016 Goals • November 3, 2016
October 2016
- Transgenerational epigenetic effects of climate stressors on oysters • October 28, 2016
- Draft Research Plan for Oyster Gonad Project • October 24, 2016
- DNA Extraction quick n' dirty (beginning steps) • October 10, 2016
- Does this thing work? • October 9, 2016