Lab day 5 - Evaporation

Evaporating - that’s it!

Goal of the day was to bring sample volumes down to ~20ul. We anticipated that this would be a 10hr process, but turned out to take 14+hrs! Here’s a timeline breakdown:

  • 9:00 AM: tooks samples to genome sciences to use their regrigerated speed-vac; someone was using it @ a different temperature, so we waited.
  • 12:30 PM: put samples on wet ice while we waited for speed vac
  • 2:00 PM: gained access to speed vac! Programmed it to: 4degC, no stop time.
  • 10:00 PM: first sample (one of Yaamini’s oyster samples) was ready for removal. Samples did not evaporate at a consistent rate.
  • When removed from speed vac, samples were put directly on wet ice, and moved to the -80 in FTR within 30 minutes.

    Sample Time Removed from Speed Vacuum
    G10 1:40 AM
    G18 1:40 AM
    G48 1:40 AM
    G58 12:00 AM
    G68 2:10 AM
    G77 2:40 AM
    G92 2:40 AM
    G97 1:40 AM
    G119 1:40 AM
    G131 3:00 AM
    GBLANK - not fully evaporated; saved remaining for next day. 3:00 AM

    Photo of the speed vac:

    speed vac photo

Written on December 13, 2016