Evaporating - that’s it!

Goal of the day was to bring sample volumes down to ~20ul. We anticipated that this would be a 10hr process, but turned out to take 14+hrs! Here’s a timeline breakdown:

• 9:00 AM: tooks samples to genome sciences to use their regrigerated speed-vac; someone was using it @ a different temperature, so we waited.
• 12:30 PM: put samples on wet ice while we waited for speed vac
• 2:00 PM: gained access to speed vac! Programmed it to: 4degC, no stop time.
• 10:00 PM: first sample (one of Yaamini’s oyster samples) was ready for removal. Samples did not evaporate at a consistent rate.

• When removed from speed vac, samples were put directly on wet ice, and moved to the -80 in FTR within 30 minutes.

  Sample	Time Removed from Speed Vacuum
  G10	1:40 AM
  G18	1:40 AM
  G48	1:40 AM
  G58	12:00 AM
  G68	2:10 AM
  G77	2:40 AM
  G92	2:40 AM
  G97	1:40 AM
  G119	1:40 AM
  G131	3:00 AM
  GBLANK - not fully evaporated; saved remaining for next day.	3:00 AM

  Photo of the speed vac: