SRM Dilution Curve Sample Prep
Prepping dilution curve samples
After we run all our samples Yaamini & I are going to run 10 samples with mixtures of geoduck/oyster protines. We will prepare them at different, known preportions of G:O, and see how the peptides we each targeted become more/less prevalent. This is good practice to prove that we are measuring what we say we are.
As Yaamini did, I pooled 5ul of 5 randomly selected samples for a total of 25ul of pooled sample - I selected samples that had the full 2ug/ul (as opposed to those that had low concentration), pipetted them into a centrifuge tube, vortexed, then spun down gently:
Then, I got Yaamini’s dilution curve samples, that already have PRTC & ACN+FA, out of the freezer, and pipetted the designated vol of my pooled geoduck sample into each tube:
Then I vortexed each, spun down gently, labeled autsampler vials, and transferred the dilutions into the vials. I put them in the -20 freezer until we are ready to use them.