QuantSeq Library Generation Batch 5
Batch 5 (larvae) plate configuration
| |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
| A |
451 |
572 |
472b |
414 |
528 |
483 |
543 |
| B |
|
|
|
|
|
|
|
| C |
403 |
461b |
431b |
564 |
532 |
481 |
491 |
| D |
|
|
|
|
|
|
|
| E |
471b |
39 |
521 |
411 |
529 |
552b |
434 |
| F |
|
|
|
|
|
|
|
| G |
401 |
513 |
441 |
444 |
44 |
B5 - NTC1 |
B5 - NTC2 |
| H |
|
|
|
|
|
|
|
| Sample No. |
[RNA] (ng/ul) |
Vol RNA used |
Vol H2O to add |
ng RNA used |
| 451 |
68.4 |
5.12 |
(0.12) |
350 |
| 572 |
LOW |
- |
5.00 |
NA |
| 472b |
97.0 |
3.61 |
1.39 |
350 |
| 414 |
168.0 |
2.08 |
2.92 |
350 |
| 528 |
87.6 |
4.00 |
1.00 |
350 |
| 483 |
99.0 |
3.54 |
1.46 |
350 |
| 543 |
162.0 |
2.16 |
2.84 |
350 |
| 403 |
136.0 |
2.57 |
2.43 |
350 |
| 461b |
84.0 |
4.17 |
0.83 |
350 |
| 431b |
83.0 |
4.22 |
0.78 |
350 |
| 564 |
152.0 |
2.30 |
2.70 |
350 |
| 532 |
93.6 |
3.74 |
1.26 |
350 |
| 481 |
89.2 |
3.92 |
1.08 |
350 |
| 491 |
58.4 |
5.00 |
- |
292 |
| 471b |
108.0 |
3.24 |
1.76 |
350 |
| 39 |
89.8 |
3.90 |
1.10 |
350 |
| 521 |
66.6 |
5.00 |
- |
333 |
| 411 |
72.6 |
4.82 |
0.18 |
350 |
| 529 |
56.8 |
5.00 |
- |
284 |
| 552b |
74.6 |
4.69 |
0.31 |
350 |
| 434 |
162.0 |
2.16 |
2.84 |
350 |
| 401 |
93.4 |
3.75 |
1.25 |
350 |
| 513 |
142.0 |
2.46 |
2.54 |
350 |
| 441 |
16.2 |
5.00 |
- |
81 |
| 444 |
70.6 |
4.96 |
0.04 |
350 |
| 44 |
41.4 |
5.00 |
- |
207 |
| B5 - NTC1 |
NA |
- |
5.00 |
NA |
| B5 - NTC2 |
NA |
- |
5.00 |
NA |
Notes
- Aliquoted 20 uL RNA into new tubes and froze in -80, except for sample #44 (aliquoted 7 uL).
Written on December 15, 2019
