We went out to Manchester on 1/25/2017 to check on the Olympia oysters that are already (!) undergoing temperature treatment, to assess the OA system.

Things we did:

• Rinsed oysters with fresh water & counted
• Filled header tanks to the brim with fresh water, dosed with 12.5% bleach (~0.5 ml / L)
• Tested Durafets in the same filtered seawater, to see how they are doing. One (the “green” one) was reading erratically.

Here is a survey of the animals, which have been in 2 temperature treatments since December 6th.

Katherine’s Oysters: (split into 2 bags per treatment)

• Katherine-SS-F2-2015-Amb: 170 live, 167 live
• Katherine-SS-F2-2015-8: 168 live, 148 live
• Grandparents (F0) are wild broodstock collected at [WHERE?] in [WHEN?], then spawned in Port Gamble hatchery.
• Parents (F1) hatched at Port Gamble, ouplanted for [X months] in [Where?]
• These (F2) hatched in 2015 and grown 100% in Clam Bay

  Jake’s Oysters: (split into 2 bags per treatment; 0 dead unless noted)

• Heare2SN2014-Amb: 51 live, 48 live
• Heare2SN2014-8: 52 live, 50 live
• Heare2NF2014-Amb: 51 live, 47 live & 1 dead
• Heare2NF2014-8: 46 live, 53 live
• Heare2HL2014-Amb: 29 live, 27 live
• Heare2HL2014-8: 28 live, 27 live

Things to do and decisions to make:

• Temperature related:
  • Need to slowly increase temperature on chilled oysters before plunging them into ambient. Increase temp 1 degree on Saturday 2/4
• What temperature to use during OA?
  • Ambient (currently 10), which warms up a couple degrees, likely getting up to 12degC.
• Ordering needs:
  • Filter cartidges from Harrington Plastics:
    • 1 micron 20” WB-20-1W
    • 5 micron 20” WB-20-5W
    • 20 micron 20” WB-20-20W
  • Reeds algae past - 1 quart shellfish diet
  • Tubing for algae dosing pumps
  • Flow meters, King acrylic
• Attach water pumps
• Set up algae dosing pumps
• Plumb at least one more valve
• Test the mixing valve; we’ll need to slowly increase temperature in all tanks for spawning, so best to test that now
• I expect we will not aerate the tanks, as this would likely mess up the chemistry. But, will the animas be okay w/o aeration; will high flow rate suffice?

Proposed OA Maintenance:

OA System Needs, during trial:

• Clean oysters & tanks 1x/week - LHS/YV
• Feed daily & clean algae header tank as needed - PSRF
• Spray down sock filters, every other day-ish - PSRF
• Replace filters, 1x/week - LHS/YV
• Keep record of weird occurrences - PSRF & LHS/YV
• Take water chemistry samples 2x/week - PSRF & LHS/YV (split once a week per)
  • 1L grab samples, measure T, S & pH (mV) with handheld probes
  • Discrete water samples dosed with HgCl, 2 per treatment & culture tank (12 total)