May 22nd, 2017
- Larval table, top row:
- Lots of bubbles on side of buckets; after speaking with PSRF this is likely a sign of supersaturated water (N or O).
- Color very light - it appears that I have the same un-even algae distribution problem on this table.
- Air stones not bubbling enough- I opened their valves a bit more and that worked. Seems like this is another depressurization issue. Not sure why.
- Lots of larvae pooled on bottom of buckets- likely due to a combination of the above issues.
- Larval table, bottom row:
- banjos very dirty, but not clogged
- air stones working well
- algae concentration appears appropriate
- Broodstock Table:
- Things appear stable!
- Very few bubbles on the sides of buckets
- Larvae present in:
- SN-10 amb B
- NF-6 amb A
- SN-6 low A
- SN-6 amb B
- NF-10 low A
- K-6 low
- K-10 amb
- Moved all buckets that were on the top shelf of the larval table to the bottom shelf. Buckets that were on top shelf were: 11, 12, 13, 16.
- Took photo of buckets 16 & 5 next to each other-obvious difference in algae concentration.
- Cleaned banjos in larvae
- Spoke with Ryan about June:
- They do not know whether staff will be working weekends through June. If they don’t, then people w/o access cards/keys won’t have access to the lab. I asked whether, in that situation, PSRF would be able/willing to work my project on weekends. Ryan said maybe Jade or Stuart could- or perhaps one of them could run my project…
- Ryan is going to talk to Stuart and consider whether Stuart or Jade could take the lead for my project while I’m at FHL. Will get an answer tomorrow.
- Counted larvae collected yesterday
- Imaged larvae collected yesterday
- Collected new larvae, sampled, counted, stocked
- Not too many larvae today. I’m streamlining this process, but currently takes a couple hours. Collection from today:
- Collected excess larvae in 2mL vials froze. Labeling scheme will be numerical:
Date, Oly, Vial # e.g. “5/22/2017 Oly 1-A”
- I had saved excess larvae from 5/20 & 5/21, so I sampled those and the excess from today:
- Determined how I should collect larval samples, and how frequently
- I will collect larval samples daily when there are >10k excess larvae
- Collection will occur after stocking buckets - I will save tripours with remaining larvae, then pull 2 samples each of 10k
- Figure out how to fix the over saturation situation
- Ryan said I could install small degassing columns on each bucket, that the water would flow through prior to entering the bucket. Hmm….
- Figure out how to fix the algae concentration situation on the larval tank - not enough space to plumb algae input further back. Options could be:
- Connect top and bottom manifold at other end to create a circular flow
- Plumb in 2 pumps for each shelf
By the way, this is my banjo setup, which works very nicely in the quick-change:
To Do Tomorrow:
- Replace drippers
- Replace banjos
- Measure dripper rate @ larval tank
- Collect water sample for algae counts from each dripper - yikes!
- Record broodstock & larvae group locations on tables/manifolds
- Screen larvae for different size classes, count, re-stock buckets:
- Screen through…. 140, 160, 180 … ?
- Sample for counts & images
- Sample for freezer
- Stock buckets - method depends on # surviving larvae
To Do with time:
- Make connection for freshwater - to do Thursday
Written on May 22, 2017