Let’s review March goals to see how I did:

Completed goals:

• Solidify personnel schedule for OA experiment through March, particularly HgCl injections during NSA conference
• Formulate plan/timeline for post-OA conditioning, spawning, setting, and rearing Oly’s. Need to do this ASAP!
• Poster - draft background/methods by 3/10, add data and have full draft by 3/17. Complete by 3/24, then attend conference!
• Draft & complete article for ComSciCon PNW, then attend
• Finalize proposal for Proposal Writing Class, study for Invert Zoology etc., also Qsci 482.

Started, but not fully completed:

• Finish Pecan run! (ETA: Monday, March 6th)
• Figure out how to use Skyline
• Run files through Skyline, then figure out what to do next!

Not Started:

• Research background/context for DNR paper; begin writing.
• Develop rough draft of Results section for DNR project

April Goals: lots to accomplish this month!

• NSF:
  • Complete activites report
  • Determine if I should go “on tenure” next year - what are the alternatives?
  • Consider appropriate timeline for applying for GRIP or GROW
  • Follow up with Marilyn about accepting Hyman & FHL funding for the Comparative Embryology course
• Proteomic project:
  • Meet with Proteomics project collaborators to discuss next steps
  • De-multiplex .raw files
  • Re-run Pecan on more powerful computer to generate full .blib file
  • Perform lit review, focus on geoduck/clams
  • Begin drafting background section
  • Develop rough draft of Geoduck Results section for DNR project
• Oly OA project:
  • Sample Oly’s post-OA on April 8th - also hold the Ladies of the Lab night out
  • Clean OA system
  • Condition Oly’s, raising temperature 1degC/day to 18deg, hold there there until 4 days before PSRF needs to reduce to 14deg, then decrease 1degC/day to 14.
  • Inventory existing spawning & rearing materials to determine what’s left to make/buy. Need: insulated 5 gal bucket, 2 gal bucket, mini banjos, air stones, blue lines, drippers (what rate?)
  • Figure out how/where to tap into heated line; can we tap in after the filtration system for PSRF’s conicals?
  • Send 4/8 histology casettes off for processing
  • Image histology slides
  • Process histology slide images with … ImageJ(?)
  • Use histology images to inform tissue sample processing
  • Research best method to use for DNA Methylation analysis; what other analyses should I consider?
  • Finalize spawning/rearing plan
  • Build system! - need to start week of 4/20
  • Augment Methods draft with Dec -> April activities
  • Get all water sampling & feeding data digitized