Arrival Inspection

• Larval table, top row:
  • Lots of bubbles on side of buckets; after speaking with PSRF this is likely a sign of supersaturated water (N or O).
  • Color very light - it appears that I have the same un-even algae distribution problem on this table.
  • Air stones not bubbling enough- I opened their valves a bit more and that worked. Seems like this is another depressurization issue. Not sure why.
  • Lots of larvae pooled on bottom of buckets- likely due to a combination of the above issues.

  

• Larval table, bottom row:
  • banjos very dirty, but not clogged
  • air stones working well
  • algae concentration appears appropriate
• Broodstock Table:
  • Things appear stable!
  • Very few bubbles on the sides of buckets
• Larvae present in:
  • SN-10 amb B
  • NF-6 amb A
  • SN-6 low A
  • SN-6 amb B
  • NF-10 low A
  • K-6 low
  • K-10 amb

Today’s Tasks:

• Moved all buckets that were on the top shelf of the larval table to the bottom shelf. Buckets that were on top shelf were: 11, 12, 13, 16.
  • Took photo of buckets 16 & 5 next to each other-obvious difference in algae concentration.
• Cleaned banjos in larvae
• Spoke with Ryan about June:
  • They do not know whether staff will be working weekends through June. If they don’t, then people w/o access cards/keys won’t have access to the lab. I asked whether, in that situation, PSRF would be able/willing to work my project on weekends. Ryan said maybe Jade or Stuart could- or perhaps one of them could run my project…
  • Ryan is going to talk to Stuart and consider whether Stuart or Jade could take the lead for my project while I’m at FHL. Will get an answer tomorrow.
• Counted larvae collected yesterday
• Imaged larvae collected yesterday
• Collected new larvae, sampled, counted, stocked
  • Not too many larvae today. I’m streamlining this process, but currently takes a couple hours. Collection from today:
• Collected excess larvae in 2mL vials froze. Labeling scheme will be numerical:

  • Date, Oly, Vial #

    e.g. “5/22/2017 Oly 1-A”

  • I had saved excess larvae from 5/20 & 5/21, so I sampled those and the excess from today:
• Determined how I should collect larval samples, and how frequently
  • I will collect larval samples daily when there are >10k excess larvae
  • Collection will occur after stocking buckets - I will save tripours with remaining larvae, then pull 2 samples each of 10k
• Figure out how to fix the over saturation situation
  • Ryan said I could install small degassing columns on each bucket, that the water would flow through prior to entering the bucket. Hmm….
• Figure out how to fix the algae concentration situation on the larval tank - not enough space to plumb algae input further back. Options could be:
  • Connect top and bottom manifold at other end to create a circular flow
  • Plumb in 2 pumps for each shelf

By the way, this is my banjo setup, which works very nicely in the quick-change:

To Do Tomorrow:

• Replace drippers
• Replace banjos
• Measure dripper rate @ larval tank
• Collect water sample for algae counts from each dripper - yikes!
• Record broodstock & larvae group locations on tables/manifolds
• Screen larvae for different size classes, count, re-stock buckets:
  • Screen through…. 140, 160, 180 … ?
  • Sample for counts & images
  • Sample for freezer
  • Stock buckets - method depends on # surviving larvae

To Do with time:

• Make connection for freshwater - to do Thursday