Assessing Oly gonad transcriptome assembly
The Trinity assembly is complete. Today I inspected it using transrate
, in addition to running blast over the weekend to annotate genes using the Uniprot/Swissprot database. Transcriptome assembly quality, as per transrate
using the trimmed/normalized reads, seem sub-par, as percent good mapping is only 10% … but I’ll investigate further. Check out my Jupyter notebook for more details: transcriptome-assess-annotate.ipynb
Written on November 8, 2018